The cash will be spent on making Langley, Taplow and Iver stations easier for passengers to access Crossrail trains when they start running in 2019.
Transport Minister Baroness Kramer said: “I am delighted to confirm the funding of these important accessibility improvements.
“This is another example of our commitment to build a world-class rail network that is open to all.
“It is only right that everyone should be able to enjoy the huge benefits that Crossrail will bring.”
The government’s investment package builds on Transport for London’s announcement last month that it would fund step-free access at Seven Kings, Maryland, Manor Park and Hanwell stations.
Now each of the 40 Crossrail stations will be ‘step free’.
Mike Brown, Managing Director of London Underground and London Rail, said: “A fully accessible transport network is a top priority for us, so we are pleased that when Crossrail is fully operational in late 2019 all 40 stations it serves will be step free, transforming how disabled people can travel in and across the city.”