The deal is worth up to £2m a year for the next two years across the East Midlands (Area 7), Yorkshire (Area 12) and the North East (Area 14).
Work will involve bridge expansion joints, waterproofing and other associated structures maintenance.
Route One director Matthew Pert said “This further cements Route One’s position as the most progressive bridges and structures contractor in the UK.
“We are looking to support A-One + moving forward in future bids for Asset Support Contracts.”