Construction Enquirer tipped Kier for the job late last year after it revealed Brookfield Multiplex had pulled out of the running.
The deal could put Kier is a strong position to ultimately build all three phases of the Ram Quarter with a total value of around £600m.
Working with project managers Gardiner & Theobald, architects EPR and engineers WSP Group, Kier will deliver 411,000 sq ft of new build housing and retail space, laid out across nine blocks of between four and ten storeys.
The first phase of the project also includes the 56,000sq ft refurbishment and remodelling of the Grade II listed brewery complex, creating restaurants, boutique shops, cafés and bars, a micro-brewery, a brewery museum and residential units.
Phase one will also see the development realign 230m of flood defences along the River Wandle in order to open up the space and provide public access along a new riverside walk.
Kier will start enabling works this month with phase one scheduled for completion in early 2017.
Haydn Mursell, chief executive, said: “Kier has extensive experience in major mixed-use and regeneration projects in London and our breadth and depth of experience has positioned us to become a partner of choice for major city regeneration schemes.”