Transport Secretary Grant Shapps today said the ambitious road building project would not go ahead after being judged not to be cost-effective.
The ambitious highways project would have linked Oxford and Cambridge via a corridor including Milton Keynes.
Development work on the project was officially paused last March to review cost benefits.
Transport Secretary Grant Shapps said: “Our analysis shows the expressway cannot deliver links that provides value for money for the taxpayer, so I have taken the decision to cancel the project.
“But we remain committed to boosting transport links in the area, helping us to create jobs and build back better from coronavirus.
“We will continue to work on more targeted, localised road improvements to boost transport in the region, alongside the transformational East West Rail, in which we have invested £760m to deliver the next phase.”